Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/03/20

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03/20/2015 Today I am digesting the ligated plasmid mini prepped yesterday (3/19/2015). I will run the digestion on a gel to see the results. Item Volume (uL) Plasmid 4.42 PstI 0.5 EcoR1 0.5 Green FD Buffer 1.0 Water 3.58 Total: 10 I also created an uncut sample to run in the gel Item Volume (uL) Plasmid 4.42 PstI 0 EcoR1 0 Loading Dye 1.67 Water 3.91 Total: 10