Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/03/20

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Today I am digesting the ligated plasmid mini prepped yesterday (3/19/2015). I will run the digestion on a gel to see the results.

Item Volume (uL)
Plasmid 4.42
PstI 0.5
EcoR1 0.5
Green FD Buffer 1.0
Water 3.58
Total: 10

I also created an uncut sample to run in the gel

Item Volume (uL)
Plasmid 4.42
PstI 0
EcoR1 0
Loading Dye 1.67
Water 3.91
Total: 10