Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/29

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mm/dd/yyyy

Transformed GG ligations from yesterday.
Theory: Maybe leaving the reaction overnight allows for more backbone colonies. When I did the first 1:10 LuxR ligation, I got no colonies on the backbone plate but when I did it for RhlR and EsaR, the backbone plate had a bunch of colonies.

Set up 2-50ul PCR reactions to make more GG-ready mCh sender vector. P097 and P130 50AT and 3:30 ext time.