Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/28

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mm/dd/yyyy Set up golden gate reactions for RhlR receiver with different concentrations of backbone to try to get as close to 0 colonies on the backbone control plates Label Bb concentration Bb ul RhlR ul pRhl ul BsmBI T4 Ligase T4 Ligase buffer H2O 1 20 fmol 0.74 1.68 2.78 0.5 1.25 2 1.05 2 10 fmol 0.37 1.68 2.78 0.5 1.25 2 1.42 3 2 fmol 0.74 of 1:10 dil 1.68 2.78 0.5 1.25 2 1.79 4 20 fmol 0.74 0 0 0.5 1.25 2 5.51 5 10 fmol 0.37 0 0 0.5 1.25 2 5.88 6 2 fmol 0.74 of 1:10 dil 0 0 0.5 1.25 2 6.25