Haynes Lab:Notebook/CRISPR Editing/2015/05/02

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05/02/2015

Gal4-EED cells, +dox for 96 hours, -dox and transfected with either KAH228 or GFP, let grow for about 12 days, transfected with g025, g032, g046, or g048 on 4/29 at 6:30pm in triplicate.
Collected cells in lysis buffer, heat for 18mins at 56deg C, put at -20

Luciferase assay

' avg std dev
Luc14 2.75 0.08
KAH228 1.02 0.06
GFP 0.76 0.01