Haynes Lab:Notebook/CRISPR Editing/2015/05/01

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05/01/2015

Cell type gRNA 260 280 260/280 ng/µL
Luc14 g051 0.213 0.107 1.993 213.13
Luc14 g051 0.16 0.079 2.012 159.928
Luc14 g052 0.196 0.097 2.011 195.786
Luc14 g052 0.189 0.096 1.974 188.894
Luc14 g053 0.305 0.152 1.997 304.55
Luc14 g053 0.121 0.063 1.938 121.418
Luc14 g054 0.168 0.084 2.005 168.386
Luc14 g054 0.138 0.068 2.02 137.513
Luc14 g055 0.17 0.086 1.97 170.326
Luc14 g055 0.201 0.102 1.977 200.799
Luc14 g056 0.092 0.047 1.96 91.739
Luc14 g056 0.176 0.089 1.986 176.135


Cell type gRNA ug DNA ul lipo 260 280 260/280 ng/µL
Gal4-EED g034 0.5 3 0.081 0.043 1.877 81.058
Gal4-EED g034 0.5 3 0.087 0.049 1.753 86.697
Gal4-EED g034 0.5 5 0.024 0.013 1.876 23.89
Gal4-EED g034 0.5 5 0.025 0.013 1.975 24.767
Gal4-EED g034 1.0 3 0.085 0.046 1.842 85.32
Gal4-EED g034 1.0 3 0.07 0.038 1.849 70.244
Gal4-EED g034 1.0 5 0.013 0.009 1.5 13.387
Gal4-EED g034 1.0 5 0.023 0.013 1.754 23.213