Haynes Lab:Notebook/CRISPR Editing/2015/03/20

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

03/20/2015

PCR of g025, g032, and g044 Luc14 and Gal4-EED+dox treated cells with Phusion and P197/198.

Nested PCR of g032 with P149/P174

98 deg for 30
30 cycles
98 for 10s
68 for 10s
72 for 15s
72 for 5 min
4 forever


Plated cells for transfections tomorrow. Gal4-EED+dox cells plated at 0.16x10^6 cells/well in 12 well plates. Luc14 cells plated at 0.1x10^6 cells / well in 12 well plates. Two plates for each cell type for a total of 4 12-well plates