Haynes Lab:Notebook/CRISPR Editing/2015/03/21

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Cells look great, about 90% confluent
Transfected, Luc14, Gal4-EED+dox for 96hours with g023, g034, g046, g048, 12 well plates, 1ug DNA/well, Lipofectamine LTX with plus reagent.

Washed Gal4-EED (+dox for 96 hours) cells in T-75 flask with PBS, replaced with media without dox.