Haynes Lab:Notebook/CRISPR Editing/2015/01/23
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Sent out untreated Luc14 gDNA PCR product of P197/P198 for sequencing:
cutting 1μg of untreated and g033 and g034 treated Luc14 cells gDNA PCRd with P197/P198 and column purified samples from 14.12.06 experiment with PfoI at 37°C for 3 hours.
Quantification with 1% agarose gel