Haynes Lab:Notebook/CRISPR Editing/2015/01/24

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01/24/2015

Cell line gRNA Rep 260 280 260/280 ng/µL
Luc14 g045 1 0.182 0.09 2.013 181.634
Luc14 g045 2 0.246 0.122 2.012 246.109
Luc14 g045 3 0.175 0.089 1.953 174.603
GAL4-EED +dox g045 1 0.074 0.036 2.058 74.461
GAL4-EED +dox g045 2 0.083 0.04 2.071 82.614
GAL4-EED +dox g045 3 0.059 0.03 1.969 58.964
Luc14 g046 1 0.255 0.125 2.036 255.228
Luc14 g046 2 0.171 0.085 2.01 170.592
Luc14 g046 3 0.144 0.072 2.003 144.251
GAL4-EED +dox g046 1 0.073 0.038 1.927 72.999
GAL4-EED +dox g046 2 0.079 0.04 1.954 78.598
GAL4-EED +dox g046 3 0.077 0.04 1.926 77.323



Plated for transfections
Used 12-well plates, have enough wells to do 3 gRNAs. Plated GAL4-EED+dox cells at 80% confluency.