Haynes Lab:Notebook/CRISPR Editing/2015/01/16

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01/16/2015 Sent out g044 PCR product for sequencing: Label Primer Direction Distance RD-1-3 P175 F 256 RD-4-6 P161 F 340 RD-7-9 P173 F 375 RD-10-12 P159 F 420 RD-13-15 P170 R 432 RD-16-18 P156 R 440 RD-19-21 P172 R 548 RD-22-24 P158 R 568 Miniprepped the other gRNA plasmids but I didn't spec them. Sample Read# 260 280 260/280 ng/µL g025 0.236 0.127 1.857 235.95 g031 0.288 0.153 1.884 288.187 g032 0.453 0.242 1.873 452.739 g048 0.345 0.184 1.875 345.075 Passaged 1:10 gal4-eed cells into T-75 flask, added 1ug/ml of dox. passaged luc14 cells 1:10.