Haynes Lab:Notebook/CRISPR Editing/2015/01/20

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

01/20/2015

induced gal4-eed cells were only at about 60-70% confluent so i split all the cells between the two 50mL conicals.


Prepared plasmids for transfection:

Sample Read# ng/µL ul for 12ug ul H2O
g023 391 30.69 29.31
g025 235.95 50.86 9.14
g031 288.187 41.64 18.36
g032 452.739 26.51 33.49
g034 302 39.74 20.26
g044 403 29.78 30.22
g045 334 35.93 24.07
g046 310 38.71 21.29
g048 345.075 34.78 25.22