Haynes Lab:Notebook/CRISPR Editing/2014/09/08

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09/08/2014

Extracted genomic DNA from CRISPR on Chromatin samples using QiaAmp kit. All concentrations look great. Samples gRNA 27 +dox rep 1 and gRNA 27 -dox rep 1 have 260/280 over 2. Did extractions in batches: first batch was 1-12, second was 13-20, and third was 21-25.

Sample Read# 260 280 260/280 ng/µL
gRNA 20 +dox rep 1 0.317 0.162 1.959 317.235
gRNA 20 +dox rep 2 0.257 0.13 1.971 256.97
gRNA 20 -dox rep 1 0.252 0.129 1.957 252.053
gRNA 20 -dox rep 2 0.186 0.098 1.896 186.423
gRNA 21 +dox rep 1 0.189 0.096 1.961 189.104
gRNA 21 +dox rep 2 0.316 0.162 1.95 315.556
gRNA 21 -dox rep 1 0.24 0.124 1.939 240.117
gRNA 21 -dox rep 2 0.228 0.121 1.891 228.478
gRNA 22 +dox rep 1 0.189 0.097 1.947 189.43
gRNA 22 +dox rep 2 0.327 0.165 1.978 326.537
gRNA 22 -dox rep 1 0.29 0.15 1.939 290.088
gRNA 22 -dox rep 2 0.293 0.157 1.869 293.275
gRNA 27 +dox rep 1 0.391 0.195 2.004 391.309
gRNA 27 +dox rep 2 0.278 0.142 1.952 277.931
gRNA 27 -dox rep 1 0.235 0.117 2.006 235.313
gRNA 27 -dox rep 2 0.206 0.105 1.964 206.214
gRNA 29 +dox rep 1 0.33 0.165 1.994 329.933
gRNA 29 +dox rep 2 0.275 0.14 1.97 275.026
gRNA 29 -dox rep 1 0.212 0.109 1.953 211.985
gRNA 29 -dox rep 2 0.2 0.103 1.942 200.274
Luc14 20 0.422 0.213 1.977 421.658
Luc14 21 0.368 0.191 1.932 368.465
Luc14 22 0.274 0.14 1.966 274.319
Luc14 27 0.221 0.115 1.924 221.046
Luc14 29 0.156 0.083 1.888 156.078