Haynes Lab:Notebook/CRISPR Editing/2014/09/05

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09/05/2014

Thoroughly checked for AmCyan using CFP filter on scope, didn't see anything.

Split all the 6-well plates into two plates. Was careful to not cross contaminate, only potential non-controlled issue would be length of trypsinization but was consistent between samples and their gal4-eed controls, but not Luc14 controls. Luc14 gRNA 20 has 5mLs media, all the rest have 4.