07/07/2014
Miniprepped the 6 colonies I picked yesterday from ligation pX330 - g14.
| Sample Read#
|
260
|
280
|
260/280
|
ng/µL
|
| Colony 1 |
2.10E-02 |
0.01 |
2.115 |
21.036
|
| Colony 2 |
1.70E-02 |
0.01 |
1.717 |
16.52
|
| Colony 3 |
1.90E-02 |
9.00E-03 |
2.105 |
18.534
|
| Colony 4 |
2.60E-02 |
1.50E-02 |
1.764 |
25.633
|
| Colony 5 |
2.20E-02 |
1.40E-02 |
1.624 |
21.991
|
| Colony 6 |
6.90E-02 |
3.70E-02 |
1.866 |
69.266
|
Cut mini preps with EcoRI and BsbI. Proper insertion of the gRNA will destroy the BsbI sites. If the colony is no cut or single cut backbone religating, I'll get bands of 5.2kb and 3.2kb. If the insert is there, I'll get one band of 8.4kb.
| '
|
Volume
|
| EcoRI |
0.5
|
| BsbI |
0.5
|
| 10X ligation buffer |
2
|
| DNA |
17
|
Ran on gel, didn't see anything. Just a faint smear for colony 6.
| 
Project name
Main project page
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