Griffitts:Protocols

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Contents

Recipes

• Common buffers
• Common media
• Stock solutions

DNA

• Phylogenetics

PCR

• PCR primers
• Standard PCR
• Overlap-extension PCR
• Arbitrary PCR
• PCR clean-up (Qiagen)
• Sequencing prep

DNA prep

• Qiagen Miniprep
• Low-copy plasmid prep
• Nucleobond Midiprep
• Genomic DNA prep (Qiagen)
• Gel electrophoresis
• Modified Eckhardt gel
• Restriction digest
• In-gel ligation

RNA

• Trizol RNA purification (bacterial)
• DNAse treatment
• Reverse transcription
• 5' RACE

Protein

• Gel recipes
• Sample buffer
• Bacterial lysis
• 6His purification
• MBP purification

Bacteria

• Media
• Antibiotics
• Tips on streaking/culturing
• Freezing procedure
• Genomic DNA prep (Qiagen)
• RNA prep (Trizol)
• ß-Gal/GUS assay
• LIVE/DEAD BacLight Staining
• Triparental Mating
• Transduction
• Chemocompetent Cells
  • Transformation
• Electrocompetent Cells
  • Transformation
• LPS Analysis
• Competition Experiments
• Modified Eckhardt gel

Plants

• Media
• Conetainer Rack Protocol
• Seed scarification and germination
• Tips for growth/seed storage
• DNA prep
• RNA prep
• Seed Storage
• Microscopy
• Nodule Bacteria Recovery
• Nodule Fixation/Embedding
• LacZ Staining of Nodules