Griffitts:Protocols

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Contents

  • 1 Recipes
  • 2 DNA
    • 2.1 PCR
    • 2.2 DNA prep
  • 3 RNA
  • 4 Protein
  • 5 Bacteria
  • 6 Plants

Recipes

  • Common buffers
  • Common media
  • Stock solutions

DNA

  • Phylogenetics

PCR

  • PCR primers
  • Standard PCR
  • Overlap-extension PCR
  • Arbitrary PCR
  • PCR clean-up (Qiagen)
  • Sequencing prep

DNA prep

  • Qiagen Miniprep
  • Low-copy plasmid prep
  • Nucleobond Midiprep
  • Genomic DNA prep (Qiagen)
  • Gel electrophoresis
  • Modified Eckhardt gel
  • Restriction digest
  • In-gel ligation

RNA

  • Trizol RNA purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5' RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification
  • MBP purification

Bacteria

  • Media
  • Antibiotics
  • Tips on streaking/culturing
  • Freezing procedure
  • Genomic DNA prep (Qiagen)
  • RNA prep (Trizol)
  • ß-Gal/GUS assay
  • LIVE/DEAD BacLight Staining
  • Triparental Mating
  • Transduction
  • Chemocompetent Cells
    • Transformation
  • Electrocompetent Cells
    • Transformation
  • LPS Analysis
  • Competition Experiments
  • Modified Eckhardt gel

Plants

  • Media
  • Conetainer Rack Protocol
  • Seed scarification and germination
  • Tips for growth/seed storage
  • DNA prep
  • RNA prep
  • Seed Storage
  • Microscopy
  • Nodule Bacteria Recovery
  • Nodule Fixation/Embedding
  • LacZ Staining of Nodules
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  • This page was last edited on 19 April 2013, at 08:23.
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