Griffitts:DNAse treatment

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  • Resuspend pellet in a mixture of 45 μL ddH2O and 5 μL NEB DNase buffer
  • Add 1 μL of DNAse I (in freezer; keep on ice)
  • Mix thoroughly
  • Place in 37°C water bath for 10 minutes
  • Add 2 μL of 0.25 M EDTA
  • Heat at 75°C for 10 minutes
  • Store sample on ice or in -20°C freezer.

When ready proceed to Reverse transcription