Griffitts:Bacterial Strains
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| Strain # | Parental strain | Plasmid | Marker | Notes |
|---|---|---|---|---|
| B001 | DH5α | pRK600 | CmR | pRK600 from MT616; demonstrated to be a functional helper strain |
| B024 | RM1021 | None | None | ΔSmb21188 (acyl-T) |
| B025 | RM1021 | None | None | ΔSmb21189 and ΔSmb21190 (glycosyltransferases) |
| B026 | RM1021 | None | None | ΔSmb21191 (msbA2) |
| B042 | RM1021 | pTB93F | None | gfp+ |
| B043 | DH5α | pXLGD4 | TcR | hemA::lacZ in pRK290 |
| B044 | DH5α | pTB93F | SpR | Ptrp::gfp S65T in pMB393 |
| B060 | MAFF303099 | None | FmR | M. loti; grows on TY-fosfomycin100 |
| B061 | XL1-Blue | pMAL-C2G | None | |
| B062 | XL1-Blue | pGEX-5X-1 | None | |
| B063 | DH5α | pGEX-4T-1 | None | |
| B068 | XL1-Blue | pDG71 | TcR, KmR | plasmid provides Ptrp:GFP (mut3) on pHC41, highly stable in planta |
| B078 | Rm1021 | pRF771 | SmR, TcR | control strain |
| B079 | MG1655 | None | None | Wild-type E. coli |
| B081 | MC4100 | None | SmR | F– araD139 Δ(argf–lac)U169* rpsL150 relA1 flb5301 fruA25 deoC1 ptsF25 e14– |
| B082 | BW25139 | None | None | pir+ recA+ |
| B083 | BW25141 | None | None | pir+ recA– (use this one for cloning R6K plasmids) |
| B084 | BW2141 | pGP704 | ApR | pGP704 is pir-dependent |
| B087 | USDA 257 | None | None | S. fredii |
| B088 | NGR234 | None | None | S. fredii |
| B089 | LBA4404 | None | None | A. tumefaciens |
| B090 | NZP2037 | None | None | M. loti |
| B091 | BR816 | None | None | R. tropici |
| B093 | USDA 110 | None | None | B. japonicum |
| B094 | MG1655 | None | SmR | transduced with lysate from MC4100 |
| B095 | DH5α | None | None | |
| B096 | DH5α | pRF771 | TcR | |
| B097 | KS1000 | None | None | from NEB |
| B098 | TB1 | None | None | from NEB |
| B099 | DH5α | pJQ200SK | None | May be HB101 instead of DH5α |
| B100 | RM1021 | None | None | |
| B103 | RM1021 | None | None | expR101 (expR+) |
| B119 | RM1021 | None | None | ΔfeuP |
| B122 | RM1021 | None | None | ΔfeuQ |
| B125 | RM1021 | None | None | trsA mutant, trsA::Tn5-110 |
| B146 | RM1021 | None | None | hisH– (interrupted by Tn5-110) |
| B147 | RM1021 | None | None | aroF– (interrupted by Tn5-110) |
| B148 | RM1021 | None | None | carA– (interrupted by Tn5-110) |
| B149 | RM1021 | None | None | ndvA– (interrupted by Tn5-110) |
| B150 | RM1021 | None | None | fixB– (interrupted by Tn5-110) |
| B151 | RM1021 | None | None | dme– (interrupted by Tn5-110) |
| B152 | RM1021 | None | None | fixI– (interrupted by Tn5-110) |
| B153 | RM1021 | None | None | exoB– (interrupted by Tn5-110) |
| B154 | RM1021 | None | None | nifK– (interrupted by Tn5-110) |
| B155 | RM1021 | None | None | thrB– (interrupted by Tn5-110) |
| B156 | RM1021 | None | None | tkt2– (interrupted by Tn5-110) |
| B157 | RM1021 | None | None | ccmB– (interrupted by Tn5-110) |
| B158 | RM1021 | None | None | exoF– (interrupted by Tn5-110) |
| B159 | RM1021 | None | None | pckA– (interrupted by Tn5-110) |
| B160 | RM1021 | None | None | carB– (interrupted by Tn5-110) |
| B161 | RM1021 | None | None | relA– (interrupted by Tn5-110) |
| B162 | RM1021 | None | None | bacA– (interrupted by Tn5-110) |
| B163 | RM1021 | None | None | eno– (interrupted by Tn5-110) |
| B164 | RM1021 | None | None | cycJ– (interrupted by Tn5-110) |
| B165 | RM1021 | None | None | unknown gene essential for symbiosis interrupted by Tn5-110 |
| B166 | RM1021 | None | None | dctA– (interrupted by Tn5-110) |
| B167 | RM1021 | None | None | phaA1– (interrupted by Tn5-110) |
| B168 | RM1021 | None | None | ilvD2– (interrupted by Tn5-110) |
| B169 | RM1021 | None | None | nodH– (interrupted by Tn5-110) |
| B170 | RM1021 | None | None | leuD– (interrupted by Tn5-110) |
| B171 | RM1021 | None | None | exoY– (interrupted by Tn5-110) |
| B172 | RM1021 | None | None | fixG– (interrupted by Tn5-110) |
| B173 | RM1021 | None | None | leuB– (interrupted by Tn5-110) |
| B174 | RM1021 | None | None | dctB– (interrupted by Tn5-110) |
| B175 | RM1021 | None | None | phoC– (interrupted by Tn5-110) |
| B176 | RM1021 | None | None | phoE– (interrupted by Tn5-110) |
| B177 | RM1021 | None | None | gltD– (interrupted by Tn5-110) |
| B178 | RM1021 | None | None | ccmC– (interrupted by Tn5-110) |
| B179 | RM1021 | None | None | purF– (interrupted by Tn5-110) |
| B180 | RM1021 | None | None | unknown gene essential for symbiosis interrupted by Tn5-110 |
| B181 | RM1021 | None | None | hisF– (interrupted by Tn5-110) |
| B182 | RM1021 | None | None | cycH– (interrupted by Tn5-110) |
| B183 | RM1021 | None | None | leuC– (interrupted by Tn5-110) |
| B184 | RM1021 | None | None | leuA– (interrupted by Tn5-110) |
| B185 | RM1021 | None | None | nifB– (interrupted by Tn5-110) |
| B186 | RM1021 | None | None | fixX– (interrupted by Tn5-110) |
| B187 | RM1021 | None | None | feuQ– (interrupted by Tn5-110) |
| B188 | RM1021 | None | None | feuP– (interrupted by Tn5-110) |
| B189 | RM1021 | None | None | fixL– (interrupted by Tn5-110) |
| B190 | RM1021 | None | None | trpE– (interrupted by Tn5-110) |
| B191 | RM1021 | None | None | purK– (interrupted by Tn5-110) |
| B192 | RM1021 | None | None | purL– (interrupted by Tn5-110) |
| B193 | RM1021 | None | None | purC– (interrupted by Tn5-110) |
| B194 | RM1021 | None | None | phoD– (interrupted by Tn5-110) |
| B195 | RM1021 | None | None | ilvI– (interrupted by Tn5-110) |
| B196 | RM1021 | None | None | exoP– (interrupted by Tn5-110) |
| B197 | RM1021 | None | None | nodB– (interrupted by Tn5-110) |
| B198 | RM1021 | None | None | cycK– (interrupted by Tn5-110) |
| B199 | RM1021 | None | None | lpsB– (interrupted by Tn5-110) |
| B200 | RM1021 | None | None | metF– (interrupted by Tn5-110) |
| B201 | RM1021 | None | None | ccmG– (interrupted by Tn5-110) |
| B202 | RM1021 | None | None | nifN– (interrupted by Tn5-110) |
| B203 | RM1021 | None | None | rpoN– (interrupted by Tn5-110) |
| B204 | RM1021 | None | None | ccsA– (interrupted by Tn5-110) |
| B205 | RM1021 | None | None | exoD– (interrupted by Tn5-110) |
| B206 | RM1021 | None | None | exoH– (interrupted by Tn5-110) |
| B207 | RM1021 | None | None | thiG– (interrupted by Tn5-110) |
| B208 | RM1021 | None | None | phaG1– (interrupted by Tn5-110) |
| B209 | RM1021 | None | None | nifE– (interrupted by Tn5-110) |
| B210 | RM1021 | None | None | ndvB– (interrupted by Tn5-110) |
| B211 | RM1021 | None | None | thiF– (interrupted by Tn5-110) |
| B212 | RM1021 | None | None | msbA2– (interrupted by Tn5-110) |
| B213 | RM1021 | None | None | purM– (interrupted by Tn5-110) |
| B214 | RM1021 | None | None | fixC– (interrupted by Tn5-110) |
| B215 | RM1021 | None | None | exoL– (interrupted by Tn5-110) |
| B216 | RM1021 | None | None | cycL– (interrupted by Tn5-110) |
| B244 | DH5α | pMAL-C2G | None | Plasmid from E. Wilson |
| B245 | RM1021 | pRF771 | SmR, TcR | |
| B250 | RM1021 | pJG230 | None | ΔgutR, reference deletion strain |
| B262 | USDA 1664* | None | None | S. medicae, good promiscuous nodulator; doesn't grow on LB—use TY |
| B263 | USDA 1652* | None | None | S. medicae, good promiscuous nodulator; doesn't grow on LB—use TY |
| B304 | RM1021 | pJG225 | NmR | |
| B306 | B119 | pJG225 | NmR | |
| B308 | B122 | pJG225 | NmR | |
| B349 | RM1021 | pJG271 | SmR, TcR | Tavr + lacZ; canonical strain for β-gal assays |
| B355 | RM1021 | pJG280 | SmR, TcR | Me1 promoter + lacZ; canonical strain for β-gal assays |
| B372 | BW25113 | pKD46 | None | λ red vector |
| B378 | DH5α | pMB393, pBBR | SpR | |
| B379 | RM1021 | pJG286 | TcR | |
| B380 | B188 | pJG286 | TcR | |
| B381 | MG1655 | None | None | lac white mutant |
| B382 | MG1655 | None | None | lac blue mutant |
| B385 | DH5α | pTE3 | TcR | |
| B386 | DH5α | pE65 | TcR | |
| B387 | DH5α | pMB89 | TcR | |
| B389 | MC4100 | pKD46 | ApR | For making λ red knockouts; grow at 30°C |
| B390 | MC4100 | pJG288 | KmR | For transposon mutagenesis |
| B392 | MG1655 | None | SmR | resistance transduced from MC4100 |
| B393 | DH5α | None | SmR | resistance arose spontaneously |
| B453 | V02675 | None | SmR, NmR | fixJ2.3::Tn5 |
| B454 | RM7095 | None | SmR, NmR | exoR95::Tn5 |
| B455 | RM7096 | None | SmR, NmR | exoS96::Tn5 |
| B456 | MB801 | None | SmR, NmR | exoX::Tn5-110 |
| B457 | MB817 | None | SmR, NmR | intergenic Tn5-110 next to syrM |
| B458 | MB846 | None | SmR, NmR | intergenic Tn5-110 next to exoY |
| B459 | MB859 | None | SmR, NmR | intergenic Tn5-110 between expD1 and expG |
*More info can be obtained for the USDA Sinorhizobium meliloti accessions here.
