User:Anthony Salvagno/Notebook/Research/2009/05/05/Gels

Lanes:

1. Marker
2. SapD (Steve Koch: Incomplete digestion?)
3. Sap14
4. Sap16
5. Sap21
6. SappBS (Steve Koch: Incomplete digestion?)
7. NotpBS (Steve Koch: Same clone as previous lane, but complete digestion)
8. NotD (Steve Koch: See lane 2; this is complete digestion with NotI as opposed to SapI)
9. Not2
10. Not16
11. Not21
12. Empty
13. Saplib
14. Notlib
15. Empty
16. Anchor

I have been naming tubes weirdly because I've been running into repetitive names. Here I have Sap# and Not# where number is the tube of which there is plasmid plus insert from a given tube (going way back) and Sap/Not is the restriction enzyme that I used to cut said tube. Sap and Notlib are libraries (after a bombing) of gDNA. See these pages:

• User:Anthony Salvagno/Notebook/Research/2009/05/01/pAS lib for library explanation (Steve Koch:I'm guessing it's double-digested EcoRI/XhoI genomic DNA, but I don't think the information was written down).
• User:Anthony Salvagno/Notebook/Research/2009/04/30/SapI Digestion of random fragments SapI digestions (lanes 2-5; not lane 6 though?)
• explanation of naming conventions
• User:Anthony Salvagno/Notebook/Research/2009/04/16/Gel Results Original shotgun clone analysis.

Lanes (inverted from picture):

1. Marker (50bp ladder)
2. Marker (50bp ladder)
3. NotHP
4. HP

HP is the hairpin adapter. So this gel didn't work well. I had to run this in a vertical acrylomide gel. I'll have to write the protocol up later.