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Version: 15 October 2015 Current Projects Combinatorial assembly of gene cassettes for cellulose degradation: Dr. Chao-Kuo Liu Conversion of green algal polysaccharides to biofuels: Alejandro Salinas-Vaccaro Enzyme engineering for cellulose degradation: Kwabena Duedu Artifical consortia for conversion of biomass to useful products: Craig Munns Arsenic Biosensor Consortium: Dr. David Radford Novel biosensor formats: Jan Oltmanns Biosensors for use in developing nations: Chris Nwanko Novel approaches to radiotherapy using engineered proteins: Dr. Jon Stanley Markerless genome editing in E. coli and related bacteria: Kuan Meng (Evan) Lee Recent Projects Novel methods for DNA assembly: PaperClip: Dr. Maryia Trubitsyna Rapid electrical detection of bacterial growth: Uros Zupancic A synthetic model system for biosynthesis of starch-like polysaccharides: Joe White Old Projects Bacteriohemerythrins: C. French Making a broad host range BioBrick vector: Edinburgh iGEM2007 team and C. French Outreach 1. Portrayal of Scientists in Science Fiction Workshops Synthetic Biology Case Study 1: development of an arsenic biosensor Synthetic Biology Case Study 2: development of processes for bioconversion of cellulosic materials Biobrick Protocols 1. Primer Design 2. Cloning parts by PCR with Pfu polymerase 2b. Cloning parts by PCR with Kod polymerase 3. Agarose Gel Electrophoresis 4. Purifying a PCR product from solution 5. Cloning a PCR product into a biobrick vector 6. Preparing and using competent E. coli cells 7. Screening colonies by PCR 8. Plasmid DNA minipreps 8b. Plasmid DNA maxipreps 9. Analytical restriction digests 10. Combining two biobricks to make a new biobrick 11. Adding a ribosome binding site to a biobrick coding sequence 12. BALTIC: a new method for combining standard BioBricks 13. MABEL: a fast easy method for removing unwanted restriction sites 14. BABEL: a new method for generating BioBricks without restriction digests. Other Protocols Growth of E. coli Growth of B. subtilis Transforming Bacillus subtilis Transforming Bacillus subtilis - 2 Plasmid DNA minipreps Restriction digests Agarose gel electrophoresis PCR Purification of DNA fragments Ligation Cloning of PCR products Preparation of cell extracts Protein Assay SDS-PAGE Native-PAGE Enrichment and growth of environmental bacteria Identification of bacteria Gram stain Making glycerol stocks Assaying polysaccharides with the anthrone assay Production of B. subtilis endospores Lab information Catalog of primers Safety Information Hazardous chemicals list Other links C. French main page Bacteriology page SBS page University of Edinburgh.