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This liquid medium is for general culture of the actinomycete Thermobifida fusca. In this recipe, cellobiose is the sole carbon source at 0.5%. Both the amount and type of carbon source may be varied; cellulose, CMC, glucose, and xylan are all acceptable sources.


For one liter liquid medium

  • Macro Salts
    • 1.5g NaCl
    • 3.1g (NH)2SO4
    • 9.1g Na2HPO4
    • 0.9g KH2HPO4
  • 0.2g Yeast Extract (optional)
  • 5.0g Cellobiose
  • 20ml [Microsalts]
  • 10ml Biotin and Thiamine


  1. Add macrosalts to 750ml diH2O
    • Heat as necessary.
  2. Adjust to 7.4 ± 0.2 pH
  3. Add additional ingredients
  4. Raise volume to 1L and cover
  5. Autoclave on liquid cycle for at least 30min.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. Remember that T. fusca is a thermophile; optimal Tgrowth is 55°C

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Relevant papers and books <biblio>

  1. Hagerdal1978 pmid=16345322



or instead, discuss this protocol.