File:T7-1 designs.pdf

T7.1 Genome Design. We split the wild-type T7 genome into six sections, alpha through zêta, using five restriction sites unique across the natural sequence. Each section shown here has a wild-type section with representations of the genetic elements: protein coding regions (blue), ribosome binding sites (purple), promoters (green), RNase III recognition sites (pink), transcription terminators (yellow), and others (gray). Elements are labeled by convention [Dunn & Studier, 1983]. Images are not to scale, but overlapping boundaries indicate elements with shared sequence. The useful natural restriction sites across each section are shown (black lines). T7.1 sections are shown below the wild-type sections. Parts are given integer numbers, 1 through 73, starting at the left end of the genome. Unique restriction site pairs bracket each part (red/blue lines, labeled D[part #]L/R). Added unique restriction sites (purple lines, U[part #]) and part length (# base pairs, open boxes) are shown.