File:T7-1 changes.pdf

Differences between wild-type T7 and T7.1. A listing from left to right on the T7 genome of the changes made during design of T7.1. Changes are shown by comparison of the annotated wild-type T7 (above) and T7.1 (below) sequences. Point mutations are capitalized. The natural ribosome binding sites are underlined. The bracketing restriction sites surrounding parts are orange (left cutter) and blue (right cutter), with overlaps of neighboring bracketing restriction sites in light green. Other features include start codons (green), stop codons (red), and overlaps of start and stop codons (purple).