File:Library qPCR example std-crv1.jpg
PhiX standard dilutions from Illumina library quantification, R2 = 0.9994. Standards diluted in TE+Tween, reactions carried out in TaqMan® Gene Expression Master Mix on an Applied Biosystems StepOnePlus, pipetted with Eppendorf Dualfilter T.I.P.S.® LoRetention tips, using Eppendorf Xplorer electronic multichannel pipets.
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