File:DNA SPRI validation example.png

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Validation done with λ phage genomic dsDNA as specified above and loaded on an Agilent Bioanalyzer DNA 1000 chip. Fragmented stock was purified with AMPure XP (3 measurement replicates) and the test sample with homemade bead mix (3 purification replicates) both at 2 volumes of bead mix. Different amounts of DNA were loaded (approximately 25 ng stock, 40 ng repurified DNA) but the size distributions are very similar. This batch of mix was successfully validated.

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current09:51, 16 November 2015Thumbnail for version as of 09:51, 16 November 20151,030 × 670 (433 KB)Joseph W. Foley (talk | contribs)Validation done with λ phage genomic dsDNA as specified above and loaded on an Agilent Bioanalyzer DNA 1000 chip. Fragmented stock was purified with AMPure XP (3 measurement replicates) and the test sample with homemade bead mix (3 purification replicate
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