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I also think that we should have a go at doing the on->off latency experiment. It would go something like this.

  1. Induce the cells as normal and wait 20 mins or so until they reach their steady level of GFP accumulation.
  2. Spin them down and resuspend them in media with no AHL.
  3. As a control, just spin them down and resuspend them in their own media.
  4. Put both sets of samples into the plate reader and measure a time course (~1hr).
  5. How long does it take for GFP synthesis rate to be within 5% of the synthesis rate of cells that went through the same spinning protocol but never had any AHL added to them.