Dandekar & Chandler:Protocols

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Media

• M9 Salts
• Selective Media
• Mueller Hinton II (Cation Adjusted)
• LB
• Kings A

General Protocols

• MIC Protocol
• Screen for Bactobolin Resistant Mutants
• AHL Extraction
• Pyocyanin Extraction
• AHL Signal Measuring
• General cloning guidelines
• Electroporation with pseudomonas or burkholderia
• Pseudomonas Mating
• Burkholderia Mating
• Colony PCR for non-E. coli strains
• PCR "sewing"

Specific Pseudomonas Projects

• Casein growth experiments
• QS induction from QS off state

Specific Bacillus Projects

• Transformation into amyE

Co-Culture Projects

• Pa/Bc Competition

Miscellaneous useful information

• Useful websites