Dandekar & Chandler:Protocols

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Contents

  • 1 Media
  • 2 General Protocols
  • 3 Specific Pseudomonas Projects
  • 4 Specific Burkholderia Projects
  • 5 Specific Bacillus Projects
  • 6 Co-Culture Projects
  • 7 Miscellaneous useful information

Media

  • M9 Salts
  • Selective Media
  • Mueller Hinton II (Cation Adjusted)
  • LB
  • Kings A

General Protocols

  • MIC Protocol
  • Screen for Bactobolin Resistant Mutants
  • AHL Extraction
  • Pyocyanin Extraction
  • AHL Signal Measuring
  • General cloning guidelines
  • Electroporation with pseudomonas or burkholderia
  • Pseudomonas Mating
  • Burkholderia Mating
  • Colony PCR for non-E. coli strains
  • PCR "sewing"

Specific Pseudomonas Projects

  • Casein growth experiments
  • QS induction from QS off state

Specific Burkholderia Projects

Specific Bacillus Projects

  • Transformation into amyE

Co-Culture Projects

  • Pa/Bc Competition

Miscellaneous useful information

  • Useful websites
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  • This page was last edited on 30 August 2016, at 11:23.
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