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Calculating Log Fold Change Values

  • Calculated the Log values for all of the Fold Change columns
  • used the equation =log(FOLD CHANGE, 2)

Reorganized data

  • Reorganized data so that all of the replicates were together so that it would be easier to compute the T-test results


  • Calculated P-values using the WT 12-week group as the comparison group
  • ran t-tests comparing that group to all of the other groups
  • used the equation in excel =ttest( selected the three WT 12 expression values , selected the three test values , 2, 3)