Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Glycerol Stock

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Preparation of Glycerol Stock for Long Term Storage of Bacteria

  • sterilize glycerol by autoclaving
  • to 1.5 ml of bacterial culture, add 0.5 ml of the sterile glycerol
  • vortex the culture to ensure that the glycerol is evenly dispersed
  • transfer the culture to a labeled storage tube
  • freeze the culture directly to -70°C for long-term storage (up to 6 months)

How to revive bacteria from glycerol stock