Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Glycerol Stock
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Preparation of Glycerol Stock for Long Term Storage of Bacteria
- sterilize glycerol by autoclaving
- to 1.5 ml of bacterial culture, add 0.5 ml of the sterile glycerol
- vortex the culture to ensure that the glycerol is evenly dispersed
- transfer the culture to a labeled storage tube
- freeze the culture directly to -70°C for long-term storage (up to 6 months)
