Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Glycerol Protocol

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Reviving Bacteria from Glycerol Stock

  • Scrape the frozen surface of the culture with a sterile inoculating needle, and then immediately streak the bacteria that adhere to the needle onto the surface of an LB agar plate containing the appropriate antibiotics. Incubate the plate overnight at 37°C
  • Scrape the frozen surface of the culture with a sterile inoculating needle or tip, and immediately immerse it in 2ml growth media within a 2059 snap-cap tube. Grow the bacteria overnight in a 37°C shaker.
  • Return the frozen culture to storage at -70°C

Source: doi:10.1101/pdb.prot4452