Christiaen:Methods

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this page provides access to the methods referred to as in the beginner's guide to ascidian developmental biology as well as other resources.

animals and embryos

sea water tank and adult animals
Most of the methods are described in details in the CSHL press - Ciona protocols, including :
dissection and fertilization
dechorionnation
Ciona electroporation
microinjection
fluorescence activated cell sorting (FACS)

molecular biology

molecular cloning
microarray analysis

immunohistochemistry and in situ hybridization

microscopy and image analysis