Calendar:IGEM:IMPERIAL/2009/Calendar:TeamProgress/2009-8-21

• Nuri Purswani 04:57, 21 August 2009 (EDT): Goals for the Drylab

• MO (linked also to M2): Investigate the effect of the secondary carbon source on bacteria.
• M1:
  • Enzyme literature review.
  • IPTG toxicity: Include effects on model and go to wetlab to get OD data
• M2: Research on modelling we can do. Also related to MO modelling.
• M3: Review killing model. See if we can find some real data.

Goals for today-

• Charles and Kun: Implement M3 killing model. Help plan IPTG experiment for next week.

• Tianyi and Nuri: -Research enzyme kinetics for cellulase and PAH.

- Effect of secondary carbon sources in MO.

Royah Vaezi
AIMS:

• Gel extract and purify RcsB, OtsA and Dam.
• Run extracts on gel with vector to determine ratios.
• Ligate inserts to vector (AK3) and leave over the weekend at 4°C.