Calendar:IGEM:IMPERIAL/2009/Calendar:TeamProgress/2009-8-11

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James Field:

Aims:

Wet lab:

  • Check plates from yesterday
  • Start cultures (5ml) for wednesday of all biobricks

Dry lab:

  • Finish colanic acid assay and SLIC cloning strategy

Results:

Wet lab:

  • Checked plates from Monday - pBAD failed to grow so re-plated. Others appeared fine.
  • Completed miniprep prep for Wednesday.


Dry lab:

  • Continued (but did not finish colanic acid assay protocol).
  • Read up on but did not design primers for SLIC.

David Roche 04:39, 11 August 2009 (EDT)
Aims:
Dry Lab:

  • Finish trehalose assay, and SLIC cloning strategy.
  • Make sure M2 is completely finished.


Aims:

  • Finish CRP-glucose promoter information: Cloning strategy
  • Promoter assay


Dineka Khurmi 05:15, 11 August 2009 (EDT)
Aims:

  • Finish promoter, Dam assays, and SLIC cloning strategy.
  • Make sure M3 is complete.


Kun Xue 05:15, 11 August 2009 (EDT)
Aims:

  • Finish restriction enzyme, Dam assays, and SLIC cloning strategy.
  • Make sure M3 is complete.

Results:

  • Did a RE and methylation assay, subject to approval
  • Put up a cloning strategy to fit multiple cloning advantage of SLIC
  • Looked through M1


Royah Vaezi
Dry Lab

  • Helped Dineka and Kun with M3 assays.

Wet Lab

  • Completed PCR of OtsB - failed to obtain bands on gel so this will be re-done.