Biomod/2014/fit test.html

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<img src="/images/4/46/Fitaologo.PNG" alt" alt="" width="422" height="98" hspace="0" align="left">



  • <a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#home">Home</a>
  • <a href="fit_Introduction.html#pro">Projects</a>
    • <a href="fit_Introduction.html#back">background & Motivation</a>
    • <a href="fit_Introduction.html#goal">Goals</a>
  • <a href="fit_Approach and Goals.html#des">Design</a>
    • <a href="fit_Approach and Goals.html#ear">Early Design</a>
    • <a href="fit_Approach and Goals.html#fin">Final Design</a>
  • <a href="fit_Method.html#met">Method</a>
    • <a href="fit_Method.html#a">Barrel particle</a>
    • <a href="fit_Method.html#b">DNA modified</a>
    • <a href="fit_Method.html#c">樽と人形の結合</a>
      • 　　　
    • <a href="fit_Method.html#d">樽人形剣</a>
      • 　　　
    • <a href="fit_Method.html#e">Material</a>
  • <a href="fit_Results and Discussion.html#">Result and Discassions</a>
    • <a href="fit_Results and Discussion.html#a">Barrel particle and Doll particle</a>
    • <a href="fit_Results and Discussion.html#b">DNA modified</a>
    • <a href="fit_Results and Discussion.html#c">樽と人形の結合</a>
    • <a href="fit_Results and Discussion.html#d">樽人形剣</a>
      • 　　　
    • <a href="fit_Results and Discussion.html#e">Conclusion</a>
  • <a href="fit_Member.html#team">Team</a>
    • <a href="fit_Member.html#men">Menber</a>
    • <a href="fit_Member.html#spo">Sponsor</a>



Abstract

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Material
Structural formula Name Manufacturer Grade
<img alt="" src="/images/2/2c/1.png.gif"/ width="160" height="100" border=""/> Sulfo-NHS DOUJINDO 200mg
<img alt="" src="/images/3/3b/FIT%EF%BC%92.png.gif" width="180" height="90" border=""/> Tetraethyl orthosilicate

(Tetraethoxysilane; TEOS)

Wako 500mg
<img alt="" src="/images/9/98/FIT%EF%BC%93.png.gif" width="180" height="90" border="" /> 3-aminopropyltriethoxysilane Wako 25g
<img alt="" src="/images/1/1f/FIT%EF%BC%94.png.png" width="230" height="120" border=""/> 1 - (3-dimethylaminopropyl) carbodiimide hydrochloride TGI 5g
<img alt="" src="/images/c/cf/FIT%E3%80%80%EF%BC%95.gif" width="150" height="100" border=""/> N-N-dimethylformamide TGI 200mg
<img alt="" src="/images/0/0a/FIT_6.gif" width="145" height="90" border=""/> Succinic anhydride Wako 25g
<img alt="" src="/images/7/73/FIT_7.gif" width="200" height="70" border=""/> Cetyltrimethylammonium bromide Wako 25g
<img alt="" src="/images/c/c2/FIT_8.gif" width="180" height="90" border=""/> MES DOUJINDO 25g




Table 3
Fluorescent moleculePlaceExcitation wavelengthMaximum fluorescence wavelength
TAMRA(red)Sword550nm580nm
FITC(green)Barrel498nm522nm




　　　　　 　　　　　 　　　　　
Apparatus
Device name Manufacturer

Model number

Measurement condition Photograph Explanations of equipment
Spectrophotometer HITACHI F-2500 WL Range:

450nm~650nm Scanning Speed: 60nm/min

<img alt="" src="/images/6/6d/FIT_9.png" width="150" height="130" border=""/> This device can see the concentration of the solution, the material characteristics, the structure of the molecule.
SEM KEYENC VE-7800 WL Range:

450nm~650nm Scanning Speed: 60nm/min

<img alt="" src="/images/9/9f/FIT_10.png" width="150" height="130" border=""> It is possible to significantly change the magnification easily. In addition, photography is possible.
Confocal laser scanning microscope

(CLSM)

Nikon ECLIPSE-Ti Ar-Laser (488nm)

Garvano mirror scanner

<img alt="" src="/images/8/8e/FIT_11.png" width="150" height="130" border="">It is possible to obtain a high resolution image in focus in the field of view for the entire sample surface with irregularities.

Three-dimensional shape measurement non-destructive, non-contact is possible






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