Biomod/2013/Titech/see more 1

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<a href="http://openwetware.org/wiki/Biomod">Biomod<a/>|<a href="http://openwetware.org/wiki/Biomod/2013">2013</a>

Design of photosensitive DNA

    We designed “Converter complex” to convert the amount of UV exposure into the amount of DNA signal. The sequence of this DNA is discribed in Fig. 1.

<img src="https://upload.wikimedia.org/wikipedia/commons/b/bf/Resolt%EF%BC%86Method_P_%E9%96%BE%E5%80%A4%E3%82%B2%E3%83%BC%E3%83%88.png"> 

 <img src="https://upload.wikimedia.org/wikipedia/commons/3/30/Resolt%EF%BC%86Method_P_%E3%81%82%E3%81%9E%E3%81%B9%EF%BD%8E%E9%85%8D%E5%88%97.png" width="372">

Fig. 1. Scheme(left) and Sequence(right) of “Converter complex”


    In the underside strand of Converter complex, five azobenzene molecules are introduced. Azobenzene is a compound composed of two phenyl rings linked by a N=N double bond, and has cis and trans isomers. It is possible to control the ratio of these two isomers by exposing azobenzene to UV of 300nm<λ<400nm (Fig. 2 ).

Fig. 2. Reaction formula of photoisomerization of azobenzene


    This dissociation reaction occurres when the introduced azobenzens isomerizes from trans-type to cis-form upon UV exposure and the hybridization with the complementary strand becomes less stable [1]. Trans-cis isomerization of azobenzene progresses according to the amount of UV exposure and dissociates the Converter complex to release DNA signal.

    As a result, by introducing Converter complex, we realizeed to convert the amount of UV exposure into the amount of DNA.

 

 

[1] Hiroyuki Asanuma, Xingguo Liang, Takayuki Yoshida, and Makoto Komiyama,;CHEMBIOCHEM 2, 39-44(2001)

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