BME100 s2016:Group7 W1030AM L1

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
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OUR TEAM

Name: Milan Patel
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LAB 1 WRITE-UP

Independent and Dependent Variables

The independent variable is the inflammation inducing agent (lipopolysaccharide). The dependent variable is the amount of protein that is produced.

Experimental Design

Groups
There will be 4 groups, each consisting of 5 people. One group will be the standard group, which will receive a dosage of 0.0mg of LPS. The second group will be given a higher dosage of LPS: 2.5 mg. The third group will be given a higher dosage than the second group: 5.0 mg LPS. Finally, the last group will be using 7.5 mg LPS. We want to measure the point at which the minimum amount of LPS causes an increase in inflammation.


Age
The subjects will be age 60+ because the study is focused on a newly discovered inflammatory protein found in the elderly.


Number of subjects per group
There will be 5 subjects in each group.




Subject Selection

Subjects will be chosen randomly as long as they meet the age requirement. A program will then generate the groups from a sample pool. The subjects must be 65 years of age and free of any other diseases that will interfere with the dosage being taken. The subjects will be divided up completely randomly using a randomization program.





Sources of Error and Bias

A possible source of error could be that one of the subjects is taking a pharmaceutical that interferes with the inflammation inducing agent. Another error could be that the method for measuring the proteins is not accurate. To control this problem, the subjects will be screened to make sure that there aren’t any factors that can interfere with the inducing agent.