# BME100 s2016:Group5 W1030AM L2

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# LAB 2 WRITE-UP

## Descriptive Statistics

Experiment 1
For humans: Mean - 0 mg - 3.834 mg Mean - 5 mg - 8.932 mg Mean - 10 mg - 61.622 mg Mean - 15 mg - 657.941 mg Standard Deviation - 0 mg - 1.523 Standard Deviation - 5 mg - 1.594 mg Standard Deviation - 10 mg - 30.111 mg Standard Deviation - 15 mg - 212.943 mg Standard Error - 0 mg - 0.482 mg Standard Error - 5 mg - 0.504 mg Standard Error - 10 mg - 9.522 mg Standard Error - 15 mg - 67.338 mg Median - 0 mg - 4.12 mg Median - 5 mg - 8.98 mg Median - 10 mg - 72.395 mg Median - 15 mg - 722.905 Mode - 0 mg - DNE Mode - 5 mg - DNE Mode - 10 mg - DNE Mode - 15 mg - DNE

Experiment 2
For rats: Mean - 0 mg - 10.516 mg Mean - 10 mg - 11.112 mg Standard Deviation - 0 mg - 2.225 mg Standard Deviation - 10 mg - 7.403 mg Standard Error - 0 mg - 0.995 mg Standard Error - 10 mg - 3.311 mg Median - 0 mg - 9.24 mg Median - 10 mg - 8.99 mg Mode - 0 mg - NONE Mode - 10 mg - NONE

Experiment 1

Experiment 2

## Analysis

Experiment 1
For the human experiment we performed an ANOVA test because we had multiple groups. The ANOVA test produced a p-value of 1.4E-16, which is less than .05 so it shows statistical significance between the groups. To follow up with this we performed a Bonferroni Correction, doing t-tests between the individual groups. The results of this were: 0mg vs 5mg = 8.6E-7, 0mg vs 10mg = 9.95E-6, 0mg vs 15mg = 1.39E-8, 5mg vs 10mg = 3.02E-5, 5mg vs 15mg = 1.57E-8, 10mg vs 15mg = 6.48E-08. Taking .05 and dividing by the number of comparisons (6) we get .00833, because all t-test values were less than .00833, we can say there was a statistically significant difference between each group comparison.

Experiment 2
For the rat experiment, we performed a t-test because we only had two groups. The t-test produced a p-value of .867, showing that there was not a statistically significant difference between the 2 groups.

## Summary/Discussion

Although we did not get statistically significant results in the rat study, we did get statistically significant results in the human study. There was a significant difference in the Inflammotin levels between each group. This tells us that the LPS treatment did increase inflammotin levels in people. it can be inferred that the Inflammotin levels would keep increasing at an exponential rate until the system reaches the body's capacity for producing the protein. according to the overall ANOVA and tests, we can be certain that the size of dosage has an impact on the levels of inflammotin in humans, but not in rats.