BME100 s2016:Group4 W1030AM L5

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Paul Gossett
Name: Desiree Yazzie
Name: Sara Gubrud
Name: Katerina Soltero
Name: Patrick Panattoni
Name: Karson Pooler


LAB 5 WRITE-UP

PCR Reaction Report

The team's experience with pipetting the samples went well. The pre-lab reading was helpful. It was easy to understand the difference between the first and second stop of the pipettor. The first stop suctions the desired amount, and the second stop puts the liquid in the tube. There was liquid left in the tubes that the DNA samples and PCR reaction mix. The team had to change the labeling scheme by placing the stickers on top of the tubes.

Fluorimeter Procedure

Web camera set-up
The program ImageJ was installed on a PC, so that it could be used to analyze the photos. A webcam was then placed in a shadowbox with a fluorimeter, and then several pictures were taken of both the SYBR Green I with the water blank, and SYBR Green I with the PCR solution.


Placing Samples onto the Fluorimeter

  1. Place SYBR Green I on a slide in the middle.
  2. Turn on the fluorimeter's excitation light.
  3. Adjust phone or webcam.
  4. Take a picture of the reaction.


Data Collection and Analysis

Images of High, Low, and Zero Calf Thymus DNA

5 μg/mL sample of Calf Thymus

5.0 calf thymus.jpg

0.5 μg/mL sample of Calf Thymus

0.5 calf thymus.jpg

0 μg/mL sample of Calf Thymus

0.0 Calf Thymus.jpg


Calibrator Mean Values Graph section 2.jpg


Calibration curves
Plot 1.jpg


Plot 2.jpg


Images of Our PCR Negative and Positive Controls


PCR Results: PCR concentrations solved

TABLE GOES HERE


PCR Results: Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL


Observed results

  • Patient 59736: Negative, Negative, Negative
  • Patient 70858: Positive, Positive, Negative


Conclusions

  • Patient 59736: Negative
  • Patient 70858: Overall positive