BME100 s2016:Group4 W1030AM L5

BME 100 Spring 2016	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Paul Gossett Name: Desiree Yazzie Name: Sara Gubrud Name: Katerina Soltero Name: Patrick Panattoni Name: Karson Pooler LAB 5 WRITE-UP PCR Reaction Report The team's experience with pipetting the samples went well. The pre-lab reading was helpful. It was easy to understand the difference between the first and second stop of the pipettor. The first stop suctions the desired amount, and the second stop puts the liquid in the tube. There was liquid left in the tubes that the DNA samples and PCR reaction mix. The team had to change the labeling scheme by placing the stickers on top of the tubes. Fluorimeter Procedure Web camera set-up The program ImageJ was installed on a PC, so that it could be used to analyze the photos. A webcam was then placed in a shadowbox with a fluorimeter, and then several pictures were taken of both the SYBR Green I with the water blank, and SYBR Green I with the PCR solution. Placing Samples onto the Fluorimeter Place SYBR Green I on a slide in the middle. Turn on the fluorimeter's excitation light. Adjust phone or webcam. Take a picture of the reaction. Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA 5 μg/mL sample of Calf Thymus 0.5 μg/mL sample of Calf Thymus 0 μg/mL sample of Calf Thymus Calibrator Mean Values Calibration curves Images of Our PCR Negative and Positive Controls PCR Results: PCR concentrations solved TABLE GOES HERE PCR Results: Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient 59736: Negative, Negative, Negative Patient 70858: Positive, Positive, Negative Conclusions Patient 59736: Negative Patient 70858: Overall positive