BME100 s2016:Group12 W1030AM L1
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LAB 1 WRITE-UP
Independent and Dependent Variables
Independent Variable: The dosage of lipopolysaccharide (LPS) is the independent variable. This is the independent variable because the dosage does not change or depend on any other factor throughout the duration of the experiment.
Dependent Variable: The amount of inflammation induced is the dependent variable. This is the dependent variable because the amount of inflammation, measured by the amount of inflammatory protein produced, depends on the dosage of LPS taken.
Groups: The experiment will be designed to consist of a minimum of three trials, and each trial will consist of a control group and a experimental group. Each group will consist of ten individuals. The control group will be given a placebo pill, and data obtained from these individuals, who have not been given any LPS, will be used as baseline. The experimental group, on the other hand, will provide data that demonstrates the correlation between the dosage of LPS received and the resulting inflammation amount. These two groups will be compared against one another so that any outlying factors present will not alter the results.
Design: Prior to the start of each trial, blood samples will be collected, and ELISA tests will be used to determine the inflammatory protein counts of each individual. After each trial has concluded, blood samples will be taken again during the time frame that the effects of LPS are at their optimal amount, and ELISA tests will be used again to measure the levels of resulting inflammatory protein.
If funding is in place, then further trials would only help to narrow down this optimal dose even further. Further trials would follow the same schematic laid out above; they would be based on the concluding results of the previous trial.
Each group will consist of ten individuals, and their ages will range between 65 and 75. The pool of candidates for this experiment will be entirely volunteers, and their selection will be randomized once a screening process has been undergone. The screening will be a relatively simple set of questions that will allow the candidate's current state of bodily health to be more accurately assessed. It will also take note of family history. Candidates that are deemed in poor health, or those that are at a high risk of unsuspecting ailments, will be dismissed. A minimum total of 60 volunteers will be needed.
Sources of Error and Bias
Potential sources of error include the following:
Depending on the variety and spread of gender and race in this experiment, results may misrepresent certain populations that were not present in sufficient amounts.