PCR Results Summary
- Our positive control PCR result was 0.13 μg/mL
- Our negative control PCR result was 0.04 μg/mL
- Patient 10088 : The images of the DNA for patient 10088 did show some of the fluorescent green dye. After splitting the color using the Image J software, the green color was definitely present. The µg/mL of Patient 10088 DNA samples were all around 0.15, which was the same value as the positive control's µg/mL value of PCR Product Concentration.
- Patient 28866 : The images of the DNA for patient 28866 showed almost none of the fluorescent green dye since the drop was almost completely blue. The color split showed that the green would not show up as clearly as it did for Patient 10088. The µg/mL of Patient 28866 DNA samples were all about 0.04, which was near the negative control's µg/mL value of PCR Product Concentration.
- Patient 10088 : The results show that Patient 10088 is positive, because the value for the PCR Product Concentration was very identical to the positive control value.
- Patient 28866 : For Patient 28866, the value of PCR Product Concentration was very close to the negative control value, which means that this patient is negative.
SNP Information & Primer Design
Background: About the Disease SNP
This disease SNP is linked to a certain protein known as lipoprotein lipase. Errors in this gene cause a number of problems, primarily relating to corruptions of proper heart function. The most noticeable phenotypic reaction to the mutation we were testing for is coronary heart disease, one of the number one killers of Americans. Although primarily linked to lifestyle and health choices, this is just one of the examples of genetic predispositions that may occur.
Primer Design and Testing
Selection of the primer is essential in order to accurately test for the DNA segment in question. We used an online resource to match a DNA segment we designed off of a description of the mutated strain. By coding a primer that includes the mutated allele, we ensure that our test is selective. If the mutation is present, the primer is capable of binding and the PCR process can proceed, rendering a positive test.