BME100 s2015:Group7 12pmL2

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
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OUR TEAM

Name: Alex Kratz
Name: Stephen Geraci
Name: Keawepono Wong
Name: Marvin Vergara
Name: Jose Rodriguez
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LAB 2 WRITE-UP

Descriptive Statistics

Experiment 1
Inflammotin Response (pg/ml):

     LPS Dosage (mg)  Mean      STDev      STError
     0                3.834     1.52       0.48
     5                8.932     1.59       0.50
     10               61.62     30.11      9.52
     15               657.94    212.93     67.34

Experiment 2
Inflammotin Response (pg/ml):

     LPS Dosage (mg)  Mean      STDev      STError
     0                10.52     2.23       1.00
     10               11.11     7.40       3.31



Results

Experiment 1 (Human)

0 mg 5 mg 10 mg 15 mg
5.23 10.72 100.19 793.17
4.23 8.46 23.46 771.45
1.86 10.19 70.87 795.09
3.67 7.29 19.27 181.27
2.98 7.54 99.65 752.78
5.83 8.67 38.37 934.23
5.24 6.15 76.26 554.87
4.27 10.92 35.25 692.23
4.01 10.09 76.98 627.65


Experiment 2 (Rats)

0 mg 10 mg
9.24 22.34
8.76 6.45
8.78 14.23
13.5 3.55
12.3 8.99



Graphing

Experiment 1

BMEgraph1.png

Experiment 2
BMEgrapg2.png





Analysis

Experiment 1 Humans
Anovahuman.png Ttesttable.png

Experiment 2 Rats
T-Test P-value: 0.867403497

Accepted P-value: p<0.5

No significant difference was shown between the two groups.

Summary/Discussion

In experiment one we see a significant increase in inflammotin production as the dosage of LPS increased. We can argue this by looking at the ANOVA test performed for the human data and seeing that the p-value found showed significant differences between the groups. The ANOVA test was performed because there were more than two groups that needed to be compared. The p-values found by the ANOVA test were significant according to the Bonferroni Correction that required p-values to be less than 0.008333.

In the second experiment we see that the rats allow for a more precise and accurate measurement of their inflammotin production levels to be taken. Yet there were only two groups of data, zero and ten milligrams of LPS, to be compared for the rats. The t-test performed on the rat data showed a p-value of 0.867. Since the p-value is above 0.5, this meant that there is no significant difference between the two groups and that increased LPS dosage did not cause increased inflammotin levels for rats. However, it is important to consider that there was only a total of ten rat subjects as opposed to the larger sample size of forty human subjects. In order to increase the variance and possibly see a better change in inflammotin for the rats, more subjects are needed for experimentation.

We were able to see a significant increase in inflammotin production levels due to higher LPS dosages only in humans.