BME100 s2015:Group6 9amL5

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Sara Belko
Name: Luc Tieu
Name: Priscilla Delgado
Name: Al Imam
Name: Francisco Campa


LAB 5 WRITE-UP

Procedure

Smart Phone Camera Settings

  • Type of Smartphone: IPhone 6
    • Flash: Off
    • ISO setting: 8
    • White Balance: Automatic
    • Exposure: Automatic
    • Saturation: Automatic
    • Contrast: Automatic


Calibration

  • Open phone's camera
  • Place upright in the cradle provided
  • Adjust the distance from phone to the sample making sure the picture is in focus and the distance is greater than 4 cm away.
Name: Set Up


  • Distance between the smart phone cradle and drop = 4.1 cm


Solutions Used for Calibration

Name: Water
Name: O.25
Name: 0.5
Name: 1
Name: 2
Name: 5



Placing Samples onto the Fluorimeter

  • 1. Pipet 80 milliliters of SYBR Green I solution in the middle of the 2 circles farthest away from the camera.
  • 2. Pipet 80 milliliters of calibration solution onto the first drop.
  • 3. Make sure the slide is lite by the blue light.


Data Analysis

Representative Images of Negative and Positive Samples

Name: Negative Sample
Name: Positive Sample

Image J Values for All Calibrator Samples


Concentration Area Mean RawIntDen Drop Raw IntDen Background Raw IntDen Drop- Background
0 582 30 17609 2428 15181
0.25 582 39.7 23098 6099 16999
0.5 582 39.6 23071 4984 18087
1 582 26.321 15319 909 14410
2 582 126.3 73506 4821 68685
5 582 112.4 65415 2478 62937


Calibration curve

Calibration6.png


PCR Results Summary

  • Our positive control PCR result was 5 μg/mL
  • Our negative control PCR result was 0 μg/mL

Observed results

  • Patient 73039 : The sample did not contain the allele so it did not glow green. The sample was observed to be 0 μg/mL.
  • Patient 40429 : The sample did contain the allele so it glowed green. The sample was observed to be 2 μg/mL.

Conclusions

  • Patient 73039 : This patient does not have coronary artery disease.
  • Patient 40429 : This patient does have coronary artery disease.



SNP Information & Primer Design

Background: About the Disease SNP

The disease SNP is associated with lipoprotein lipase, which helps regulate phospholipase activity. It caused by a genetic mutation in the 8th chromosome. More specifically, in the 5' strand, changing AAT to AGT. As a result, it causes coronary artery disease in homo sapiens. Coronary artery disease, or heart disease, is one of the deadliest diseases in America.

Primer Design and Testing

At the end of the primer test, the non-diseased primer test gave a 220bp reading, while the diseased primer had no match.


Name: Nondieased
Name: Diseased