BME100 s2015:Group6 12pmL2

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OUR TEAM

Name: Justin Blommer
Name: Ethan Mathew
Name: Spencer Cobb
Name: Sarah Jones
Name: Abigail Rene

LAB 2 WRITE-UP

What is the lowest possible dose of lipopolysaccharide (LPS) that will increase Inflammotin levels in the elderly?

Descriptive Statistics

Rat Study

0mg Group

Mean: 10.516 pg/ml

Standard Deviation: 2.226 pg/ml

Standard Error: 0.995 pg/ml


10mg Group

Mean: 11.112 pg/ml

Standard Deviation: 7.403 pg/ml

Standard Error: 3.310 pg/ml


Human Study

0mg Group

Mean: 3.834 pg/ml

Standard Deviation: 1.523 pg/ml

Standard Error: 0.482 pg/ml


5mg Group

Mean: 8.932 pg/ml

Standard Deviation: 1.594 pg/ml

Standard Error: 0.504 pg/ml


10mg Group

Mean: 61.622 pg/ml

Standard Deviation: 30.111 pg/ml

Standard Error: 9.521 pg/ml


15mg Group

Mean: 657.941 pg/ml

Standard Deviation: 212.943 pg/ml

Standard Error: 67.338 pg/ml



Results

LPS Rat Study

Rat2.jpg


LPS Human Study

Human.jpg




Analysis

Rat Study

For the rat study, the T-Test was used because there were only 2 groups. When the test was ran, it gave the following value:

P-Value= 0.867403497

Since the p-value is greater than 0.05, there is no significant statistical difference between the two groups.


Human Study

For the human study, the One-Way ANOVA test was used because there were more than two groups being compared.

Anova5.jpg


Post-Test

Pt66.jpg

To establish the Bonferroni Correction, the Alpha Value (.05) was divided by six (the number of post-test comparisons).

Then, the p-values were compared to the Bonferroni Correction. Since all of the post-test p-values were smaller than the Bonferroni Correction, there was a significant statistical difference for each of the comparisons.




Summary/Discussion

In the study on rats, the LPS had a minimal impact on the Inflammotin levels. The p-value from the T-test was 0.867, indicating that it was not statistically significant. When the same study was conducted on humans, the LPS dosage had a much greater impact on subjects' Inflammotin levels. The p-value from the ANOVA test was 1.40*10^-16, indicating that it was statistically significant. From the results, it can be concluded that the LPS impacted the Inflammotin levels in humans much more than it did in rats.