BME100 s2015:Group6 12pmL1

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Justin Blommer Name: Ethan Mathew Name: Spencer Cobb Name: Sarah Jones Name: Abigail Rene LAB 1 WRITE-UP What is the lowest possible dose of lipopolysaccharide (LPS) that can increase Inflammotin levels in the elderly? Independent and Dependent Variables Independent Variable- Lipopolysaccharide (LPS) Dependent Variable- Inflammotin Experimental Design Groups Control Groups: 0mg of LPS 10 mg of LPS Experimental Groups: Dosage of LPS for the 9 experimental groups: 1mg of LPS 2mg of LPS 3mg of LPS 4mg of LPS 5mg of LPS 6mg of LPS 7mg of LPS 8mg of LPS 9mg of LPS Age All of our test subjects will be in the 70-75 year old range. We chose a more precise age range because a 70 year old and a 90 year old could have extremely different results in our experiment. If order to determine effectiveness of the LPS in older subjects, we would perform the same experiment with an older test group. Number of subjects per group -10 subjects per group -Groups will be 5 male and 5 female -Aside from gender, all subjects will be randomly chosen in terms of race, age (within the 70-75 range), cultural background, etc. Subject Selection -All subjects must be 70-75 years old -Should have a similar health history, but otherwise diverse (race, culture, gender, etc) -Patients should not already have inflammation issues or be taking anti-inflammatory drugs Sources of Error and Bias Error: Health history and previous medical conditions may affect how subject's body reacts to LPS Solution: Must select test subjects without inflammation issues and of similar health history Error: Subjects with a tolerance or extreme sensitivity to LPS will react to it differently Solution: Ensure subjects are not currently taking anti-inflammatory medicines and that their other medications will not interfere with test results Error: Genetics can alter subject's tolerance and how their body responds to LPS Solution: Select as diverse of a test group as possible Error: Body size/composition affects metabolization Solution: Choose subjects with similar body compositions Error: Using the same test group could produce biased Inflammotin levels due to residual LPS in the system from previous trial Solution: Each trial uses a different group of test subjects Error: Test subjects may have different baseline levels of Inflammotin prior to receiving LPS Solution: Select subjects that had similar baseline levels