BME100 s2015:Group5 9amL2

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OUR TEAM

Name: Jacob Licko
Name: Daniela Romero
Name: Stephen Belikoff
Name: Khalil Khoury
Name: Wei Lu

LAB 2 WRITE-UP

Descriptive Statistics

Humans

Dosage (mg) 0 5 10 15
Average Inflammotin Levels (pg/mL) 3.384 8.932 61.622 657.941
Standard Deviation 1.523010177 1.59393155 30.11069386 212.942976
Standard Error 0.481618106 0.50404541 9.521837451 67.3384817

Rat

Dosage (mg) 0 10
Average Inflammotin Levels (pg/mL) 10.516 11.112
Standard Deviation 2.225551617 7.402885924
Standard Error 0.995296941 3.310671231




Results

Experiment 1
Human Graph.PNG


Experiment 2
Rat Graph.PNG




Analysis

Experiment 1
ANOVA1.PNG Table A

T-testsResults.PNG Table B

For the human trials there were more than 2 groups, which called for the ANOVA test. Once the ANOVA test resulted in a p-value less than 0.05, as shown in table A, it was determined that there was a significant differentce betweent the groups. Now, in order to compare the 4 groups by pairs, t-tests were used for 6 different comparisons, as shown in table B. Because there is an error involved with multiple comparisons, the Bonferroni correction was used to adjust the p-value that the t-test values must be less than for there to be a significant difference. In this case, there were 6 total comparisons and so the normal p-value was divided by 6, (0.05/6), to get the value 8.33E-03. Each t-test value was less than 8.33E-03 and so there was a significance with each comparison, as shown in table B.



Experiment 2
Ratttest.PNG Table C

For the rat trials, since there wre only two groups and only one comparison, the t-test was a much more appropriate choice. As shown in table C, the two-tail p-value was not less than 0.05 and so there was no significant difference. It is possible that the drug, LPS, has little to no effect in rats compared to humans.




Summary/Discussion

The human trials showed that there was a significant difference between the groups of different dosages. This information leads to the conclusion that the dosage of LPS has a direct effect on the amount of inflammotin, as the dosage goes up so does inflammation. However, that was not the case with the rats. Although there was a small increase in inflammotin with an increase in dosage, it was not enough to be considered significantly different. The rat trials could have lacked significant difference bacasue rats do not react to the drug in the same way that humans do.