BME100 s2015:Group5 12pmL1
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LAB 1 WRITE-UP
Independent and Dependent Variables
Independent Variable: Amount (mg) of LPS
Dependent Variable: Activity of Inflammotin
3mg Experimental Group
5mg Experimental Group
7mg Experimental Group
Measurement of Inflammotin Activity
Subjects will have their blood drawn twice per week: once immediately prior to LPS administration, and again one hour after LPS administration. This will allow for a comparison between baseline Inflammotin levels and experimental Inflammotin levels.
Subjects will undergo LPS administration and subsequent blood drawing for three weeks, with one week passing between each LPS administration (i.e. Wednesday, following Wednesday, following Wednesday). Experiment will last 3 weeks.
Healthy (No pre-existing conditions)
Not currently taking any other medications
Haven't taken any medications for the month leading up to the study
No family history of inflammation
Moderate exercise history (at least 30 mins a day)
Subjects must be able to walk
No medical allergies
Sources of Error and Bias
Genetic factors inherent to each patient could skew the results, with some of the patients metabolizing the drug more efficiently than others. Lifestyle choices such as dietary choices or daily activities may also affect the data. Subjects may also be unaware of their family medical history, which could cause for unsuspected results. Finally, various environmental factors, like outside temperature or humidity, could potentially affect the activity of inflammotin. All of these are being mitigated through larger sample size and multiple trials.
Confirmation bias is a huge potential for skewing results, as experimenters will be seeking an effect, which will change the interpretation of the datum.