BME100 s2015:Group5 12pmL1

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Brandon Favre Name: Nick Valverde Name: Martisha Clyde Name: Daniella Orlando Name: David Shumate LAB 1 WRITE-UP Independent and Dependent Variables Independent Variable: Amount (mg) of LPS Dependent Variable: Activity of Inflammotin Experimental Design Groups 10mg Control Group 3mg Experimental Group 5mg Experimental Group 7mg Experimental Group Age Participants will be above the age of 60 Number of subjects per group 25 people per group. Measurement of Inflammotin Activity The enzyme-linked immunosorbant assay (ELISA) will be used to determine the presence of inflammotin. Subjects will have their blood drawn twice per week: once immediately prior to LPS administration, and again one hour after LPS administration. This will allow for a comparison between baseline Inflammotin levels and experimental Inflammotin levels. Subjects will undergo LPS administration and subsequent blood drawing for three weeks, with one week passing between each LPS administration (i.e. Wednesday, following Wednesday, following Wednesday). Experiment will last 3 weeks. Subject Selection Healthy (No pre-existing conditions) Not currently taking any other medications Haven't taken any medications for the month leading up to the study No family history of inflammation Moderate exercise history (at least 30 mins a day) Subjects must be able to walk No medical allergies Sources of Error and Bias Genetic factors inherent to each patient could skew the results, with some of the patients metabolizing the drug more efficiently than others. Lifestyle choices such as dietary choices or daily activities may also affect the data. Subjects may also be unaware of their family medical history, which could cause for unsuspected results. Finally, various environmental factors, like outside temperature or humidity, could potentially affect the activity of inflammotin. All of these are being mitigated through larger sample size and multiple trials. Confirmation bias is a huge potential for skewing results, as experimenters will be seeking an effect, which will change the interpretation of the datum.