BME100 s2015:Group3 9amL1
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LAB 1 WRITE-UP
Independent and Dependent Variables
The independent variable would be the dosage, measure in milligrams, of the inflammatory drug (lipopolysaccharide), because this variable is purposefully changed during the experiment. The dependent variable would be the protein levels, measured using blood samples, because the protein levels are affected by the change in lipopolysaccharide dosage.
Three groups of ten will be tested to determine the lowest possible dose of lipopolysaccharide to increase a newly discovered inflammatory protein found in the elderly. Each group will consist of 5 males and 5 females. Subjects of group 1 will be given a placebo to measure natural levels of Inflammotin, this will be the control group. Group 2 will be given 5 mg of lipopolysaccharide and group 3 will be given 10 mg of lipopolysaccharide.
10 subjects per group to provide variability for testing date, but no more than 10 test subjects due to insufficient funds for research.
- Elderly home volunteers with no underlining health conditions.
- Volunteers who are not taking anti-inflammatory medications.
- Ages 60-85 years old.
Sources of Error and Bias
We have five sources of error. Diet, prior medication, and genetics.