BME100 s2015:Group14 12pmL2
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LAB 2 WRITE-UP
Experiment 1 - Rats
Experiment 2 – Human Study
For a study in rats, a t-test was performed to determine the significance of the data found in the experiment. With the results from the t-test, it was concluded that there was no significant difference in rats who received 0 mg of LPS and 10 mg of LPS. Inflammotin levels were not affected. For the study in humans, an ANOVA was performed and a corrected p-value was used to ensure that all results were accurate. T-tests between each group were also performed to determine the significance of different levels of LPS dosage. The results of the ANOVA and the t-tests showed that in humans, LPS dosage increases inflammotin production with increasing dosage.
Possible reasons for the inconsistency between the rat and the humans study are as follows: - There were only two test groups involved in the rat study, and dosages of inflammotin were either 0 mg or 10 mg. - Rats may not be affected by LPS. They might have a different inflammotin production mechanism which isn't enhanced by LPS. They might also not produce inflammotin.
To ensure more significant results for rats, perhaps a wider range of dosages of LPS can be used. Also, a greater number of test groups can be involved. Additionally, more study should go into seeing if rats have different mechanisms for producing inflammotin. If an animal study is not effective for a critical drug, then most manufacturers probably would not want to jump in on a human study. Animal studies are important preliminaries in testing any drug or device.