# BME100 s2015:Group14 12pmL1

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# OUR TEAM

 Name: Nathan Francois Name: Lexi Bounds Name: Dakota Graham Name: Thor Kissman Name: Justin Mieth

# LAB 1 WRITE-UP

## Independent and Dependent Variables

- Independent variable = LPS dosage

- Dependent variable = inflammotin levels in blood

## Experimental Design

Question: What is the minimum dosage of LPS required to increase levels of the protein inflammotin in the elderly?

Hypothesis: There is a lesser dosage than 10 mg that produces the desired levels of protein.

Prediction: If a subject takes a 6 mg dosage of LPS, then they will produce the desired levels of inflammotin.

Procedure: Two groups of 10 people,5 male and 5 female, will be selected based on a similar BMI and age of 65-70. One group will start with a control dosage of 0 mg and their dosage will increase by 2 mg every 2 weeks. They will be given a week of the dosage, and then will have their inflammotin levels measured. After this, they will be given a placebo for the next week. After a week of the placebo, they will return to receive their next dosage and will have inflammotin levels measured. The second group will follow a similar pattern, but will start at a beginning dosage of 1 mg. Over the course of 12 weeks, the dosages will be gradually increased for both groups, the levels of inflammotin will be measured at the end of each week, and the final dosage for each group will be 10 mg for trial 6.

Groups

2 groups will be used for the experiment.

Age

Ages 65 to 70 years old.

Number of subjects per group

10 subjects per group, 5 male and 5 female.

## Subject Selection

Individuals will be selected at random from a pool of subjects with a similar Body Mass Index, age, and a low risk medical history. Individuals must have a BMI within the healthy range, and be between 65 and 70 years old.

## Sources of Error and Bias

- Unequal diet and exercise among subjects

- genetics

- unknown medical conditions affecting inflammotin

- vitamin and mineral deficiencies

- medical emergencies

- Improper following of instructions by test subjects

- allergies

- small sample size

- limited sample from a very specific age range and medical background