BME100 s2015:Group11 12pmL1
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LAB 1 WRITE-UP
Independent and Dependent Variables
Independent Variable: Amount of LPS (lipopolysaccharide; mg)
LPS is the independent variable because the level of LPS is what causes the change, (Inflammotin levels), in the experiment.
Dependent Variable: Amount of Inflammotin (mg)
The amount of Inflammotin is dependent on the amount of LPS. It is the variable that is being changed over the course of the experiment.
Men (Experimental), Women (Experimental), Men (Control), Women (Control)
We want as random of a selection as possible for elderly participants. We will screen out participants with chronic illness. The reason we have groups for male and female is to control for the physiological differences between the sexes.
65-70 years old
We chose the age range of 65-70 because it is considered elderly but has less negative health conditions than their older elderly counterparts. The age range of five years is a realistic number to get a large enough sample size.
15 people per group
We chose 15 participants per group because it is a realistic sample size for our budget and provides variety. We would administer the LPS in different levels each week and measure the Inflammatonresulting from it/
Subjects will be selected based on public advertisements made in various forms of media, (Internet, newspapers, flyers). Each subject will be compensated for their time.
Sources of Error and Bias
Any pre-existing medical conditions that might influence the response of subjects to LPS may be a source of error. In addition, subject lifestyle, such as diet, exercise, smoking/drinking preferences may affect how the participant responds to the drug. Environmental factors, such as temperature, elevation, pollution, and stress may also skew the response toward the LPS.
In order to account for these potential errors, the experiment already screens out for individuals with known pre-existing conditions. Additionally, to remove any error from environmental factors, the experiment would select individuals within a certain city or area to ensure all subjects are exposed to the same environment. Lastly, the experiment would have potential participants take a survey to account for lifestyle choices that may impact health and choose subjects that appear to meet the criteria of the experiment for healthy men and women.