BME100 f2013:W900 Group9 L1
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LAB 1 WRITE-UP
Independent and Dependent Variables
The independent variable is the dosage of the lipopolysaccharide (measured in mg).
Number of subjects per group
All subjects will be volunteers between the ages of 60 and 80 years old. Each group will contain subjects with different ethnic backgrounds, living conditions and lifestyles, and gender. Also, the subjects cannot be taking any anti-inflammatory medication. Each group should be as diverse as possible to determine if the dosage of lipopolysaccharide will increase Inflammotin levels in a majority of the elderly population.
Sources of Error and Bias
Several biases and errors may occur throughout the experiment. Subjects between groups will vary genetically, yielding different results for each patient. This includes a variety of factors; ethnic/geographic background, blood type, age, and gender. This may affect their responses to the lipopolysaccharide, and a specific genetic trait may also be responsible for how the patient responds. Additionally, each patient is likely to have a different lifestyle, and will have different choices in diet and exercise. Other medications - aside from the prohibited anti-inflammatory drugs - that the patients are taking may also interfere with the experiment.
Errors surrounding the experimental design itself may also be present. For example, the true minimum amount of lipopolysaccharide needed for inducing inflammation may lie between two values tested (such as if the minimum amount was 9 mg, the experiment would not yield that result because only 8 and 10 mg were tested). This could be fixed by running a second experiment with smaller dosage increments once a general range is established from the first experiment.